Professional Design High Quality Proteinase K CAS No. 39450-01-6 ndi Mtengo Wabwino Kwambiri
Nthawi zambiri timagwira ntchito yowoneka bwino ndikuwonetsetsa kuti tikupatseni zabwino kwambiri kuphatikiza mtengo wabwino kwambiri wogulitsa wa Professional Design High QualityProteinase K CAS No. 39450-01-6ndi Mtengo Wabwino Kwambiri, Kuti mumve zambiri komanso zowona, chonde lankhulani nafe mwachangu momwe mungathere!
Nthawi zambiri timagwira ntchito yogwirika ndikuwonetsetsa kuti tikukupatsani zabwino kwambiri komanso mtengo wabwino kwambiri wogulitsaChina Tritirachium Album Limber ndi Molecular Biology, Ulamuliro wokhwima wa khalidwe umagwiritsidwa ntchito mu chiyanjano chilichonse cha ndondomeko yonse yopangira.Kutengera mayankho apamwamba kwambiri komanso ntchito yabwino yogulitsira / kugulitsa pambuyo pake ndi lingaliro lathu, makasitomala ena adagwirizana nafe kwa zaka zopitilira 5.
| No.1 madzi | |
| Zochita zamapuloteni> 800U/mL, ndende yake ndi 20mg/mL, palibe zotsalira za Nickase. | |
| No.2 ufa | |
| Maonekedwe | White lyophilized ufa |
| Electrophoretic chiyero | ≥95% |
| Ntchito ya enzyme | ≥30U/mg |
| Nucleic acid zotsalira | Zosaoneka |
| Deoxyribonuclease zotsalira | Zosaoneka |
| Ribonuclease zotsalira | Zosaoneka |
| Ngolo No. | Chithunzi cha G1205-10ML |
| Kusungirako | Akasungidwa pa -20 ° C, mankhwalawa amasunga ntchito kwa zaka zosachepera 1, zoyendera za ayezi. |
Proteinase Kndi serine protease wa m'banja la subtilisin omwe ali ndi mphamvu yogwira ntchito ya enzymatic komanso yotakata gawo lapansi, yomwe makamaka imawola ma ester bond ndi peptides moyandikana ndi C-terminus ya hydrophobic amino acid, ma amino acid okhala ndi sulfure, ndi zomangira za amino acid onunkhira. Nthawi zambiri amagwiritsidwa ntchito kuwononga mapuloteni kuti apange ma peptides afupi.Proteinase Kili ndi mawonekedwe amtundu wa catalytic triad Asp39-His69-Ser224 wapadera kwa serine proteases ndipo ili ndi malo awiri omangirira a Ca2+ kuzungulira likulu logwira ntchito kuti awonjezere kukhazikika kwake ndikukhalabe ndi zochita zambiri za enzymatic pansi pamikhalidwe yambiri.
1. Zida zodziwira chibadwa.
2. RNA ndi DNA m'zigawo zida.
3. Tulutsani zigawo zopanda mapuloteni mu minofu ndi kuwononga zonyansa zamapuloteni (monga kukonzekera katemera wa DNA ndi heparin).
4. Kukonzekera kwa Chromosomal DNA kwa Pulse Electrophoresis.
5. Kutseka chakumadzulo.
6. Kukula ndi kupanga ma enzymatic glycated albumin reagents m'munda wa in vitro diagnostics.
1. 30mg/botolo 1g/botolo kapena 100g/botolo
2. 1kgs/thumba la zojambulazo
Proteinase K ndi serine protease yokhazikika yokhala ndi gawo lalikulu la gawo lapansi.Imawononga mapuloteni ambiri m'dziko lakwawo ngakhale pamaso pa zotsukira.Simayendetsedwa ndi ayoni achitsulo, ma chelating agents (mwachitsanzo, EDTA), ma reagents a sulfhydryl, kapena ndi tripsin kapena chymotrypsin inhibitors.Ndiwokhazikika pa pH yamitundumitundu (4-12.5), yokhala ndi ntchito yabwino pa pH 6.5-9.5.Zochita zitha kukopeka powonjezera ma denaturing agents (SDS ndi Urea).Kuthamanga kwachangu kwa enzyme kumachitika pa kutentha pamwamba pa 70 ° C.Autolysis ya enzyme imapezeka kwambiri pa pH ya alkaline.Komabe, Proteinase K sichimachotsedwa kwathunthu ndi autolysis.Zidutswa zina za ma enzyme zimapitilizabe kugwira ntchito yawo yonse ya proteolytic, ngakhale pambuyo popanga autolysis.Proteinase K nthawi zambiri amagwiritsidwa ntchito popanga mapuloteni osafunika, monga ma nucleases mu DNA kapena RNA kukonzekera kuchokera ku tizilombo tating'onoting'ono, maselo otukuka, ndi zomera.Enzyme amagwiritsidwa ntchito pa 50-200 ug/ml mu nucleic acid kukonzekera pa pH 7.5-8.0 ndi 37 ~ 55oC.Nthawi ya incubation imasiyanasiyana kuyambira mphindi 30 mpaka maola 18.
A khola ndi zotakasuka kwambiri serine protease;amagwiritsidwa ntchito popanga mapuloteni ndi nucleic acid isolation.Proteinase K imagwiritsidwa ntchito poyeretsa RNA ndi DNA kuchokera ku minofu kapena mizere ya maselo.
Nthawi zambiri timagwira ntchito yogwirika ndikuwonetsetsa kuti tikupatseni zopindulitsa kwambiri kuphatikiza mtengo wabwino kwambiri wogulitsa wa Professional Design High Quality Proteinase K.CAS No. 39450-01-6ndi Mtengo Wabwino Kwambiri, Kuti mumve zambiri komanso zowona, chonde lankhulani nafe mwachangu momwe mungathere!
Professional DesignChina Tritirachium Album Limber ndi Molecular Biology, Ulamuliro wokhwima wa khalidwe umagwiritsidwa ntchito mu chiyanjano chilichonse cha ndondomeko yonse yopangira.Kutengera mayankho apamwamba kwambiri komanso ntchito yabwino yogulitsira / kugulitsa pambuyo pake ndi lingaliro lathu, makasitomala ena adagwirizana nafe kwa zaka zopitilira 5.
