ODM Factory Proteinase K CAS No. 39450-01-6 Yolimbikitsa Kulimbitsa Mafupa
Ntchito yathu ndikutumikira ogwiritsa ntchito ndi makasitomala athu ndi zinthu zabwino kwambiri komanso zopikisana za digito za ODM Factory Proteinase K CAS No. mayankho abwino komanso kutumiza mwachangu. Tikukhulupirira kuti mutha kutipatsa mwayi wokupatsani ndikukhala bwenzi lanu lapamtima!
Ntchito yathu ndikutumikira ogwiritsa ntchito ndi makasitomala athu ndi zinthu zabwino kwambiri komanso zopikisana nazo za digitoChina Chemicals Product ndi Raw Zipangizo, Iwo ndi olimba owonetsera ndi kulimbikitsa bwino padziko lonse lapansi. Osasowa ntchito zazikulu munthawi yachangu, ndizofunikira kwa inu panokha zamtundu wabwino kwambiri. Motsogozedwa ndi mfundo ya Prudence, Efficiency, Union and Innovation. bungwe. yesetsani kukulitsa malonda ake apadziko lonse, kukweza bungwe lake. rofit ndikukweza kukula kwake kwa katundu wotumiza kunja. Takhala ndi chidaliro kuti tidzakhala ndi chiyembekezo chowoneka bwino ndikufalitsidwa padziko lonse lapansi m'zaka zikubwerazi.
| No.1 madzi | |
| Zochita zamapuloteni> 800U/mL, ndende yake ndi 20mg/mL, palibe zotsalira za Nickase. | |
| No.2 ufa | |
| Maonekedwe | White lyophilized ufa |
| Electrophoretic chiyero | ≥95% |
| Ntchito ya enzyme | ≥30U/mg |
| Nucleic acid zotsalira | Zosaoneka |
| Deoxyribonuclease zotsalira | Zosaoneka |
| Ribonuclease zotsalira | Zosaoneka |
| Ngolo No. | Chithunzi cha G1205-10ML |
| Kusungirako | Akasungidwa pa -20 ° C, mankhwalawa amasunga ntchito kwa zaka zosachepera 1, zoyendera za ayezi. |
Proteinase K ndi serine protease wa banja la subtilisin yokhala ndi ma enzymatic amphamvu komanso yotakata gawo lapansi, yomwe imakonda kuwola ma ester bond ndi peptides moyandikana ndi C-terminus ya hydrophobic amino acid, ma amino acid okhala ndi sulfure, ndi ma amino acid onunkhira, zomwe nthawi zambiri zimagwiritsidwa ntchito kusokoneza mapuloteni kuti apange ma peptides afupi. Proteinase K ili ndi mawonekedwe amtundu wa catalytic triad Asp39-His69-Ser224 yapadera ku serine proteases ndipo ili ndi malo awiri omangirira a Ca2+ kuzungulira likulu logwira ntchito kuti awonjezere kukhazikika kwake ndikusunga ma enzymatic ambiri pansi pamikhalidwe yochulukirapo.
1. Zida zodziwira chibadwa.
2. RNA ndi DNA m'zigawo zida.
3. Tulutsani zigawo zopanda mapuloteni m'magulu ndi kuwononga zonyansa zamapuloteni (monga kukonzekera katemera wa DNA ndi heparin).
4. Kukonzekera kwa Chromosomal DNA kwa Pulse Electrophoresis.
5. Kutseka kwa Kumadzulo.
6. Kukula ndi kupanga ma enzymatic glycated albumin reagents m'munda wa in vitro diagnostics.
1. 30mg/botolo 1g/botolo kapena 100g/botolo
2. 1kgs/thumba la zojambulazo
Proteinase K ndi serine protease yokhazikika yokhala ndi gawo lalikulu la gawo lapansi. Imawononga mapuloteni ambiri m'dziko lakwawo ngakhale pamaso pa zotsukira. Simayendetsedwa ndi ayoni achitsulo, ma chelating agents (mwachitsanzo, EDTA), ma reagents a sulfhydryl, kapena ndi tripsin kapena chymotrypsin inhibitors. Ndiwokhazikika pa pH yamitundumitundu (4-12.5), yokhala ndi ntchito yabwino pa pH 6.5-9.5. Zochita zitha kukopeka powonjezera ma denaturing agents (SDS ndi Urea). Kuthamanga kwachangu kwa enzyme kumachitika pa kutentha pamwamba pa 70 ° C. Autolysis ya enzyme imapezeka kwambiri pa pH ya alkaline. Komabe, Proteinase K sichimachotsedwa kwathunthu ndi autolysis. Zidutswa zina za ma enzyme zimapitilizabe kugwira ntchito yawo yonse ya proteolytic, ngakhale pambuyo popanga autolysis. Proteinase K nthawi zambiri amagwiritsidwa ntchito popanga mapuloteni osafunika, monga ma nucleases mu DNA kapena RNA kukonzekera kuchokera ku tizilombo tating'onoting'ono, maselo otukuka, ndi zomera. Enzyme amagwiritsidwa ntchito pa 50-200 ug/ml mu nucleic acid kukonzekera pa pH 7.5-8.0 ndi 37 ~ 55oC. Nthawi ya incubation imasiyanasiyana kuyambira mphindi 30 mpaka maola 18.
A khola ndi zotakasuka kwambiri serine protease; amagwiritsidwa ntchito popanga mapuloteni ndi nucleic acid isolation.Proteinase K amagwiritsidwa ntchito poyeretsa RNA ndi DNA kuchokera ku minofu kapena mizere ya maselo.
Ntchito yathu ndikutumikira ogwiritsa ntchito ndi makasitomala athu ndi zinthu zabwino kwambiri komanso zopikisana za digito za ODM Factory Proteinase K CAS No. mayankho abwino komanso kutumiza mwachangu. Tikukhulupirira kuti mutha kutipatsa mwayi wokupatsani ndikukhala bwenzi lanu lapamtima!
ODM FactoryChina Chemicals Product ndi Raw Zipangizo, Iwo ndi olimba owonetsera ndi kulimbikitsa bwino padziko lonse lapansi. Osasowa ntchito zazikulu munthawi yachangu, ndizofunikira kwa inu panokha zamtundu wabwino kwambiri. Motsogozedwa ndi mfundo ya Prudence, Efficiency, Union and Innovation. bungwe. yesetsani kukulitsa malonda ake apadziko lonse, kukweza bungwe lake. rofit ndikukweza kukula kwake kwa katundu wotumiza kunja. Takhala ndi chidaliro kuti tidzakhala ndi chiyembekezo chowoneka bwino ndikufalitsidwa padziko lonse lapansi m'zaka zikubwerazi.
