Mbiri Yapamwamba Kugulitsa Ma Proteinase K CAS 39450-01-6 Quality Guaranteed
Timaganiza zomwe makasitomala amaganiza, kufulumira kuchitapo kanthu mkati mwa zofuna za kasitomala wa mfundo zofunika kwambiri, kulola kuti zikhale bwino kwambiri, kuchepetsa ndalama zoyendetsera ntchito, zolipiritsa ndizowonjezera zomveka, zinapambana makasitomala atsopano ndi apitawo thandizo ndi kutsimikizira kwa Mbiri yapamwamba Yogulitsa KutenthaProteinase K CAS 39450-01-6Quality Guaranteed, Monga katswiri wodziwa ntchito imeneyi, tadzipereka kuthetsa vuto lililonse la chitetezo cha kutentha kwa ogwiritsa ntchito.
Timaganiza zomwe makasitomala amaganiza, kufulumira kuchitapo kanthu mkati mwa zofuna za kasitomala wa mfundo zofunika kwambiri, kulola kuti zikhale bwino kwambiri, kuchepetsa ndalama zoyendetsera ntchito, zolipiritsa ndizowonjezera zomveka, zinapambana makasitomala atsopano ndi apitawo thandizo ndi kutsimikizira kwaChina Proteinase K ndi CAS 39450-01-6, Pali zida zapamwamba zopangira & kukonza ndi antchito aluso kuonetsetsa kuti zinthuzo ndi zapamwamba kwambiri.Tapeza ntchito yabwino kwambiri tisanagulitse, kugulitsa, kugulitsa pambuyo potsimikizira makasitomala omwe angakhale otsimikizika kuti apanga maoda.Mpaka pano mayankho athu akuyenda mwachangu komanso otchuka kwambiri ku South America, East Asia, Middle East, Africa, etc.
| No.1 madzi | |
| Zochita zamapuloteni> 800U/mL, ndende yake ndi 20mg/mL, palibe zotsalira za Nickase. | |
| No.2 ufa | |
| Maonekedwe | White lyophilized ufa |
| Electrophoretic chiyero | ≥95% |
| Ntchito ya enzyme | ≥30U/mg |
| Nucleic acid zotsalira | Zosaoneka |
| Deoxyribonuclease zotsalira | Zosaoneka |
| Ribonuclease zotsalira | Zosaoneka |
| Ngolo No. | Chithunzi cha G1205-10ML |
| Kusungirako | Akasungidwa pa -20 ° C, mankhwalawa amasunga ntchito kwa zaka zosachepera 1, zoyendera za ayezi. |
Proteinase Kndi serine protease wa m'banja la subtilisin omwe ali ndi mphamvu yogwira ntchito ya enzymatic komanso yotakata gawo lapansi, yomwe makamaka imawola ma ester bond ndi peptides moyandikana ndi C-terminus ya hydrophobic amino acid, ma amino acid okhala ndi sulfure, ndi zomangira za amino acid onunkhira. Nthawi zambiri amagwiritsidwa ntchito kuwononga mapuloteni kuti apange ma peptides afupi.Proteinase K ili ndi mawonekedwe amtundu wa catalytic triad Asp39-His69-Ser224 yapadera ku serine proteases ndipo ili ndi malo awiri omangirira a Ca2+ kuzungulira likulu logwira ntchito kuti awonjezere kukhazikika kwake ndikusunga ma enzymatic ambiri pansi pamikhalidwe yochulukirapo.
1. Zida zodziwira chibadwa.
2. RNA ndi DNA m'zigawo zida.
3. Tulutsani zigawo zopanda mapuloteni mu minofu ndi kuwononga zonyansa zamapuloteni (monga kukonzekera katemera wa DNA ndi heparin).
4. Kukonzekera kwa Chromosomal DNA kwa Pulse Electrophoresis.
5. Kutseka chakumadzulo.
6. Kukula ndi kupanga ma enzymatic glycated albumin reagents m'munda wa in vitro diagnostics.
1. 30mg/botolo 1g/botolo kapena 100g/botolo
2. 1kgs/thumba la zojambulazo
Proteinase K ndi serine protease yokhazikika yokhala ndi gawo lalikulu la gawo lapansi.Imawononga mapuloteni ambiri m'dziko lakwawo ngakhale pamaso pa zotsukira.Simayendetsedwa ndi ayoni achitsulo, ma chelating agents (mwachitsanzo, EDTA), ma reagents a sulfhydryl, kapena ndi tripsin kapena chymotrypsin inhibitors.Ndiwokhazikika pa pH yamitundumitundu (4-12.5), yokhala ndi ntchito yabwino pa pH 6.5-9.5.Zochita zitha kukopeka powonjezera ma denaturing agents (SDS ndi Urea).Kuthamanga kwachangu kwa enzyme kumachitika pa kutentha pamwamba pa 70 ° C.Autolysis ya enzyme imapezeka kwambiri pa pH ya alkaline.Komabe, Proteinase K sichimachotsedwa kwathunthu ndi autolysis.Zidutswa zina za ma enzyme zimapitilizabe kugwira ntchito yawo yonse ya proteolytic, ngakhale pambuyo popanga autolysis.Proteinase K nthawi zambiri amagwiritsidwa ntchito popanga mapuloteni osafunika, monga ma nucleases mu DNA kapena RNA kukonzekera kuchokera ku tizilombo tating'onoting'ono, maselo otukuka, ndi zomera.Enzyme amagwiritsidwa ntchito pa 50-200 ug/ml mu nucleic acid kukonzekera pa pH 7.5-8.0 ndi 37 ~ 55oC.Nthawi ya incubation imasiyanasiyana kuyambira mphindi 30 mpaka maola 18.
A khola ndi zotakasuka kwambiri serine protease;amagwiritsidwa ntchito popanga mapuloteni ndi nucleic acid isolation.Proteinase K imagwiritsidwa ntchito poyeretsa RNA ndi DNA kuchokera ku minofu kapena mizere ya maselo.
Timaganiza zomwe makasitomala amaganiza, kufulumira kuchitapo kanthu mkati mwa zofuna za kasitomala wa mfundo zofunika kwambiri, kulola kuti zikhale bwino kwambiri, kuchepetsa ndalama zoyendetsera ntchito, zolipiritsa ndizowonjezera zomveka, zinapambana makasitomala atsopano ndi apitawo thandizo ndi kutsimikizira kwa Mkulu mbiri Hot Selling Proteinase K CAS39450-01-6Quality Guaranteed, Monga katswiri wodziwa ntchito imeneyi, tadzipereka kuthetsa vuto lililonse la chitetezo cha kutentha kwa ogwiritsa ntchito.
Mbiri yapamwambaChina Proteinase K ndi CAS 39450-01-6, Pali zida zapamwamba zopangira & kukonza ndi antchito aluso kuonetsetsa kuti zinthuzo ndi zapamwamba kwambiri.Tapeza ntchito yabwino kwambiri tisanagulitse, kugulitsa, kugulitsa pambuyo potsimikizira makasitomala omwe angakhale otsimikizika kuti apanga maoda.Mpaka pano mayankho athu akuyenda mwachangu komanso otchuka kwambiri ku South America, East Asia, Middle East, Africa, etc.
