fakitale mtengo wotsika Alkaline ProteinaseK CAS 39450-01-6

Tadzipereka kupereka mpikisano, zinthu zapadera ndi mayankho apamwamba, komanso kutumiza mwachangu pamtengo wotsika wamafuta amchereProteinaseK CAS 39450-01-6, Takhala tikufunitsitsa mtsogolo kuti tigwirizane ndi ogula kulikonse padziko lapansi. Tikukhulupirira kuti tidzakukhutiritsani. Timalandilanso mwachikondi ogula kuti apite ku bungwe lathu ndikugula zinthu zathu.
Tadzipereka kupereka mtengo wampikisano, zinthu zapadera ndi mayankho apamwamba kwambiri, komanso kutumiza mwachangu kwaChina Alkaline ProteinaseK ndi 39450-01-6, Chifukwa cha zabwino komanso mitengo yabwino, malonda athu atumizidwa kumayiko ndi zigawo zopitilira 10. Tikuyembekezera kugwirizana ndi makasitomala onse ochokera kunyumba ndi kunja. Komanso, kukhutira kwamakasitomala ndikofuna kwathu kosatha.

Proteinase Kndi serine protease wa m'banja la subtilisin omwe ali ndi mphamvu yogwira ntchito ya enzymatic komanso yotakata gawo lapansi, yomwe makamaka imawola ma ester bond ndi peptides moyandikana ndi C-terminus ya hydrophobic amino acid, ma amino acid okhala ndi sulfure, ndi zomangira za amino acid onunkhira. Nthawi zambiri amagwiritsidwa ntchito kuwononga mapuloteni kuti apange ma peptides afupi. Proteinase K ili ndi mawonekedwe amtundu wa catalytic triad Asp39-His69-Ser224 yapadera ku serine proteases ndipo ili ndi malo awiri omangirira a Ca2+ kuzungulira likulu logwira ntchito kuti awonjezere kukhazikika kwake ndikusunga ma enzymatic ambiri pansi pamikhalidwe yochulukirapo.

1. Zida zodziwira chibadwa.
2. RNA ndi DNA m'zigawo zida.
3. Tulutsani zigawo zopanda mapuloteni m'magulu ndi kuwononga zonyansa zamapuloteni (monga kukonzekera katemera wa DNA ndi heparin).
4. Kukonzekera kwa Chromosomal DNA kwa Pulse Electrophoresis.
5. Kutseka kwa Kumadzulo.
6. Kukula ndi kupanga ma enzymatic glycated albumin reagents m'munda wa in vitro diagnostics.

1. 30mg/botolo 1g/botolo kapena 100g/botolo
2. 1kgs/thumba la zojambulazo

Proteinase K ndi serine protease yokhazikika yokhala ndi gawo lalikulu la gawo lapansi. Imawononga mapuloteni ambiri m'dziko lakwawo ngakhale pamaso pa zotsukira. Simayendetsedwa ndi ayoni achitsulo, ma chelating agents (mwachitsanzo, EDTA), ma reagents a sulfhydryl, kapena ndi tripsin kapena chymotrypsin inhibitors. Ndiwokhazikika pa pH yamitundumitundu (4-12.5), yokhala ndi ntchito yabwino pa pH 6.5-9.5. Zochita zitha kukopeka powonjezera ma denaturing agents (SDS ndi Urea). Kuthamanga kwachangu kwa enzyme kumachitika pa kutentha pamwamba pa 70 ° C. Autolysis ya enzyme imapezeka kwambiri pa pH ya alkaline. Komabe, Proteinase K sichimachotsedwa kwathunthu ndi autolysis. Zidutswa zina za ma enzyme zimapitilizabe kugwira ntchito yawo yonse ya proteolytic, ngakhale pambuyo popanga autolysis. Proteinase K nthawi zambiri amagwiritsidwa ntchito popanga mapuloteni osafunika, monga ma nucleases mu DNA kapena RNA kukonzekera kuchokera ku tizilombo tating'onoting'ono, maselo otukuka, ndi zomera. Enzyme amagwiritsidwa ntchito pa 50-200 ug/ml mu nucleic acid kukonzekera pa pH 7.5-8.0 ndi 37 ~ 55oC. Nthawi ya incubation imasiyanasiyana kuyambira mphindi 30 mpaka maola 18.

A khola ndi zotakasuka kwambiri serine protease; amagwiritsidwa ntchito popanga mapuloteni ndi nucleic acid isolation.Proteinase K amagwiritsidwa ntchito poyeretsa RNA ndi DNA kuchokera ku minofu kapena mizere ya maselo.

Tadzipereka kupereka mpikisano, zinthu zapadera ndi mayankho apamwamba kwambiri, komanso kutumiza mwachangu pamtengo wotsika fakitale Alkaline ProteinaseKCAS39450-01-6, Takhala tikufunitsitsa mtsogolo kuti tigwirizane ndi ogula kulikonse padziko lapansi. Tikukhulupirira kuti tidzakukhutiritsani. Timalandilanso mwachikondi ogula kuti apite ku bungwe lathu ndikugula zinthu zathu.
mtengo wotsika wa fakitaleChina Alkaline ProteinaseK ndi 39450-01-6, Chifukwa cha zabwino komanso mitengo yabwino, malonda athu atumizidwa kumayiko ndi zigawo zopitilira 10. Tikuyembekezera kugwirizana ndi makasitomala onse ochokera kunyumba ndi kunja. Komanso, kukhutira kwamakasitomala ndikofuna kwathu kosatha.