Mtengo wotsika wa fakitale Alkaline ProteinaseK CAS 39450-01-6
Tikudzipereka kupereka mtengo wopikisana, zinthu zabwino kwambiri komanso mayankho abwino kwambiri, komanso kutumiza mwachangu pamtengo wotsika wa Alkaline m'fakitale.ProteinaseK CAS 39450-01-6, Takhala tikufunitsitsa kwambiri kugwirizana ndi ogula padziko lonse lapansi. Tikukhulupirira kuti tidzakukhutiritsani. Timalandiranso ogula kuti abwere ku bungwe lathu ndikugula zinthu zathu.
Tikudzipereka kupereka mtengo wampikisano, zinthu zabwino kwambiri komanso mayankho abwino kwambiri, komanso kutumiza mwachangu kwaChina Alkaline ProteinaseK ndi 39450-01-6Chifukwa cha khalidwe labwino komanso mitengo yabwino, katundu wathu watumizidwa kumayiko ndi madera opitilira 10. Tikuyembekezera kugwirizana ndi makasitomala onse ochokera kunyumba ndi kunja. Kuphatikiza apo, kukhutitsidwa kwa makasitomala ndiye cholinga chathu chosatha.
| Madzi nambala 1 | |
| Ntchito yeniyeni ya mapuloteni> 800U/mL, kuchuluka kwake ndi 20mg/mL, palibe zotsalira za Nickase | |
| Ufa wa nambala 2 | |
| Maonekedwe | Ufa woyera wophilizidwa |
| Kuyera kwa electrophoretic | ≥95% |
| Ntchito ya enzyme | ≥30U/mg |
| zotsalira za nyukiliya | Zosaoneka |
| Zotsalira za Deoxyribonuclease | Zosaoneka |
| Zotsalira za Ribonuclease | Zosaoneka |
| Nambala ya Ngolo Yogulitsira | G1205-10ML |
| Malo Osungirako | Mukasungidwa pa -20 °C, mankhwalawa amasunga ntchito kwa chaka chimodzi, osapitirira chaka chimodzi. |
Mapuloteni Kndi serine protease yomwe ili m'gulu la subtilisin lomwe lili ndi ntchito yothandiza ya enzymatic komanso broad substrate specificity, yomwe imakonda kuwononga ma ester bond ndi ma peptides omwe ali pafupi ndi C-terminus ya ma amino acids a hydrophobic, ma amino acid okhala ndi sulfur, ndi ma amino acid bond onunkhira, omwe nthawi zambiri amagwiritsidwa ntchito kuwononga mapuloteni kuti apange ma peptides afupi. Proteinase K ili ndi mawonekedwe a catalytic triad wamba Asp39-His69-Ser224 yapadera kwa ma serine proteases ndipo ili ndi malo awiri omangira a Ca2+ kuzungulira malo ogwirira ntchito kuti awonjezere kukhazikika kwake ndikusunga ntchito yayikulu ya enzymatic pansi pa mikhalidwe yosiyanasiyana.
1. Zida zodziwira matenda a majini.
2. Zida zochotsera RNA ndi DNA.
3. Chotsani zinthu zopanda mapuloteni m'thupi ndikuwononga zinthu zodetsa mapuloteni (monga kukonzekera katemera wa DNA ndi heparin).
4. Kukonzekera DNA ya Chromosomal kuti igwiritsidwe ntchito pa Pulse Electrophoresis.
5. Kufalikira kwa Western.
6. Kupanga ndi kupanga ma enzyme glycated albumin reagents m'munda wa in vitro diagnostics.
1. 30mg/botolo 1g/botolo kapena 100g/botolo
2. 1kgs/thumba la zojambulazo
Proteinase K ndi protease yokhazikika ya serine yokhala ndi mawonekedwe apadera a substrate. Imawononga mapuloteni ambiri omwe ali m'malo mwake ngakhale pakakhala sopo. Siimagwira ntchito ndi ma ayoni achitsulo, ma chelating agents (monga EDTA), ma sulfhydryl reagents, kapena ndi tripsin kapena chymotrypsin inhibitors. Ndi yokhazikika pa pH yochuluka (4–12.5), yokhala ndi ntchito yabwino kwambiri pa pH 6.5–9.5. Ntchito imatha kuyambitsidwa powonjezera ma denaturing agents (SDS ndi Urea). Denaturation yofulumira ya enzyme imachitika kutentha kopitilira 70°C. Autolysis ya enzyme imachitika kwambiri pa alkaline pH. Komabe, Proteinase K siigwira ntchito kwathunthu ndi autolysis. Zidutswa zina za enzyme zimapitirizabe kugwira ntchito yawo yonse ya proteolytic, ngakhale pambuyo pa autolysis yayikulu. Proteinase K imagwiritsidwa ntchito nthawi zambiri mu ma molecular biology kuti idye mapuloteni osafunikira, monga ma nucleases mu DNA kapena RNA preparations ochokera ku tizilombo toyambitsa matenda, maselo obzalidwa, ndi zomera. Enzymeyi nthawi zambiri imagwiritsidwa ntchito pa 50–200 ug/ml mu nucleic acid yokonzekera pa pH 7.5–8.0 ndi 37~55oC. Nthawi yofukizira imasiyana kuyambira mphindi 30 mpaka maola 18.
Proteinase K imagwiritsidwa ntchito poyeretsa RNA ndi DNA kuchokera ku minofu kapena maselo.
Tikudzipereka kupereka zinthu zabwino kwambiri, zotsika mtengo komanso mayankho abwino kwambiri, komanso kutumiza mwachangu pamtengo wotsika wa Alkaline ProteinaseK kuchokera ku fakitale.CAS39450-01-6, Takhala tikufunitsitsa kwambiri kugwirizana ndi ogula padziko lonse lapansi. Tikukhulupirira kuti tidzakukhutiritsani. Timalandiranso ogula kuti abwere ku bungwe lathu ndikugula zinthu zathu.
mtengo wotsika wa fakitaleChina Alkaline ProteinaseK ndi 39450-01-6Chifukwa cha khalidwe labwino komanso mitengo yabwino, katundu wathu watumizidwa kumayiko ndi madera opitilira 10. Tikuyembekezera kugwirizana ndi makasitomala onse ochokera kunyumba ndi kunja. Kuphatikiza apo, kukhutitsidwa kwa makasitomala ndiye cholinga chathu chosatha.
