Fakitale yomwe ikugulitsidwa kwambiri Proteinase K CAS 39450-01-6 yokhala ndi Kuwonekera Kwambiri komanso Tsiku Laposachedwa Lopanga
Membala aliyense payekhapayekha kuchokera ku gulu lathu lalikulu lomwe limagwira ntchito amayamikira zomwe makasitomala amafuna komanso kulumikizana ndi bungwe pa Factory yomwe ikugulitsidwa bwino kwambiri Padziko LonseProteinase K CAS 39450-01-6ndi High Exposure Rate ndi Tsiku Laposachedwa Lopanga, Panopa, tikuyembekezera mgwirizano wokulirapo ndi makasitomala akunja kutengera mapindu omwewo. Onetsetsani kuti simukumva kuti mulibe mtengo kuti mulumikizane nafe kuti mudziwe zambiri.
Membala aliyense payekhapayekha kuchokera ku gulu lathu lalikulu lomwe amapeza ndalama amayamikira zomwe makasitomala amafuna komanso kulumikizana ndi bungweChina 39450-01-6, Tikulandirani mwachikondi kuthandizidwa kwanu ndipo tidzatumikira makasitomala athu kunyumba ndi kunja ndi katundu wapamwamba kwambiri ndi ntchito zabwino kwambiri zomwe zimagwirizana ndi chitukuko monga nthawi zonse. Tikukhulupirira kuti mudzapindula ndi ukatswiri wathu posachedwa.
| No.1 madzi | |
| Zochita zamapuloteni> 800U/mL, ndende yake ndi 20mg/mL, palibe zotsalira za Nickase. | |
| No.2 ufa | |
| Maonekedwe | White lyophilized ufa |
| Electrophoretic chiyero | ≥95% |
| Ntchito ya enzyme | ≥30U/mg |
| Nucleic acid zotsalira | Zosaoneka |
| Deoxyribonuclease zotsalira | Zosaoneka |
| Ribonuclease zotsalira | Zosaoneka |
| Ngolo No. | Chithunzi cha G1205-10ML |
| Kusungirako | Akasungidwa pa -20 ° C, mankhwalawa amasunga ntchito kwa zaka zosachepera 1, zoyendera za ayezi. |
Proteinase Kndi serine protease wa m'banja la subtilisin omwe ali ndi mphamvu yogwira ntchito ya enzymatic komanso yotakata gawo lapansi, yomwe makamaka imawola ma ester bond ndi peptides moyandikana ndi C-terminus ya hydrophobic amino acid, ma amino acid okhala ndi sulfure, ndi zomangira za amino acid onunkhira. Nthawi zambiri amagwiritsidwa ntchito kuwononga mapuloteni kuti apange ma peptides afupi. Proteinase K ili ndi mawonekedwe amtundu wa catalytic triad Asp39-His69-Ser224 yapadera ku serine proteases ndipo ili ndi malo awiri omangirira a Ca2+ kuzungulira likulu logwira ntchito kuti awonjezere kukhazikika kwake ndikusunga ma enzymatic ambiri pansi pamikhalidwe yochulukirapo.
1. Zida zodziwira chibadwa.
2. RNA ndi DNA m'zigawo zida.
3. Tulutsani zigawo zopanda mapuloteni m'magulu ndi kuwononga zonyansa zamapuloteni (monga kukonzekera katemera wa DNA ndi heparin).
4. Kukonzekera kwa Chromosomal DNA kwa Pulse Electrophoresis.
5. Kutseka kwa Kumadzulo.
6. Kukula ndi kupanga ma enzymatic glycated albumin reagents m'munda wa in vitro diagnostics.
1. 30mg/botolo 1g/botolo kapena 100g/botolo
2. 1kgs/thumba la zojambulazo
Proteinase K ndi serine protease yokhazikika yokhala ndi gawo lalikulu la gawo lapansi. Imawononga mapuloteni ambiri m'dziko lakwawo ngakhale pamaso pa zotsukira. Simayendetsedwa ndi ayoni achitsulo, ma chelating agents (mwachitsanzo, EDTA), ma reagents a sulfhydryl, kapena ndi tripsin kapena chymotrypsin inhibitors. Ndiwokhazikika pa pH yamitundumitundu (4-12.5), yokhala ndi ntchito yabwino pa pH 6.5-9.5. Zochita zitha kukopeka powonjezera ma denaturing agents (SDS ndi Urea). Kuthamanga kwachangu kwa enzyme kumachitika pa kutentha pamwamba pa 70 ° C. Autolysis ya enzyme imapezeka kwambiri pa pH ya alkaline. Komabe, Proteinase K sichimachotsedwa kwathunthu ndi autolysis. Zidutswa zina za ma enzyme zimapitilizabe kugwira ntchito yawo yonse ya proteolytic, ngakhale pambuyo popanga autolysis. Proteinase K nthawi zambiri amagwiritsidwa ntchito popanga mapuloteni osafunika, monga ma nucleases mu DNA kapena RNA kukonzekera kuchokera ku tizilombo tating'onoting'ono, maselo otukuka, ndi zomera. Enzyme amagwiritsidwa ntchito pa 50-200 ug/ml mu nucleic acid kukonzekera pa pH 7.5-8.0 ndi 37 ~ 55oC. Nthawi ya incubation imasiyanasiyana kuyambira mphindi 30 mpaka maola 18.
A khola ndi zotakasuka kwambiri serine protease; amagwiritsidwa ntchito popanga mapuloteni ndi nucleic acid isolation.Proteinase K amagwiritsidwa ntchito poyeretsa RNA ndi DNA kuchokera ku minofu kapena mizere ya maselo.
Membala aliyense payekhapayekha kuchokera ku gulu lathu lalikulu lomwe amapeza ndalama amawona zomwe makasitomala amafuna komanso kulumikizana ndi bungwe pa Factory yomwe ikugulitsidwa bwino kwambiri padziko lonse lapansi Proteinase K CAS39450-01-6ndi High Exposure Rate ndi Tsiku Laposachedwa Lopanga, Panopa, tikuyembekezera mgwirizano wokulirapo ndi makasitomala akunja kutengera mapindu omwewo. Onetsetsani kuti simukumva kuti mulibe mtengo kuti mulumikizane nafe kuti mudziwe zambiri.
Kugulitsa kwambiri fakitaleChina 39450-01-6, Tikulandirani mwachikondi kuthandizika kwanu ndipo tidzatumikira makasitomala athu kunyumba ndi kunja ndi katundu wapamwamba kwambiri ndi ntchito zabwino kwambiri zomwe zimagwirizana ndi chitukuko monga nthawi zonse. Tikukhulupirira kuti mudzapindula ndi ukatswiri wathu posachedwa.
