Proteinase K CAS 39450-01-6 yogulitsidwa kwambiri padziko lonse lapansi yokhala ndi chiwopsezo chachikulu cha kufalikira kwa khansa komanso tsiku lomaliza la kupangidwa kwake.
Membala aliyense wa gulu lathu lalikulu lopeza ndalama zogwirira ntchito amayamikira zosowa za makasitomala ndi kulumikizana kwa bungwe kuti agulitse kwambiri ku Fakitale Yotchuka Padziko LonseProteinase K CAS 39450-01-6Ndi chiwopsezo chachikulu cha kufalikira kwa zinthu komanso tsiku lomaliza la kupanga, pakadali pano, tikuyembekezera mgwirizano waukulu ndi makasitomala akunja kutengera phindu lomwe tonse tili nalo. Onetsetsani kuti simukulipira chilichonse kuti mulumikizane nafe kuti mudziwe zambiri.
Membala aliyense wa gulu lathu lalikulu lopeza ndalama zogwirira ntchito amayamikira zosowa za makasitomala ndi kulumikizana kwa bungwe kwaChina 39450-01-6, Tikulandirani ndi manja awiri thandizo lanu ndipo tidzatumikira makasitomala athu kunyumba ndi kunja ndi zinthu zapamwamba kwambiri komanso ntchito yabwino kwambiri yogwirizana ndi chitukuko chamtsogolo monga mwa nthawi zonse. Tikukhulupirira kuti mudzapindula ndi ukatswiri wathu posachedwa.
| Madzi nambala 1 | |
| Ntchito yeniyeni ya mapuloteni> 800U/mL, kuchuluka kwake ndi 20mg/mL, palibe zotsalira za Nickase | |
| Ufa wa nambala 2 | |
| Maonekedwe | Ufa woyera wophilizidwa |
| Kuyera kwa electrophoretic | ≥95% |
| Ntchito ya enzyme | ≥30U/mg |
| zotsalira za nyukiliya | Zosaoneka |
| Zotsalira za Deoxyribonuclease | Zosaoneka |
| Zotsalira za Ribonuclease | Zosaoneka |
| Nambala ya Ngolo Yogulitsira | G1205-10ML |
| Malo Osungirako | Mukasungidwa pa -20 °C, mankhwalawa amasunga ntchito kwa chaka chimodzi, osapitirira chaka chimodzi. |
Mapuloteni Kndi serine protease ya banja la subtilisin yokhala ndi ntchito yothandiza ya enzymatic komanso kudziwika bwino kwa substrate, yomwe imawononga ma ester bond ndi ma peptides omwe ali pafupi ndi C-terminus ya ma amino acids a hydrophobic, ma amino acid okhala ndi sulfure, ndi ma amino acid bond onunkhira, omwe nthawi zambiri amagwiritsidwa ntchito kuwononga mapuloteni kuti apange ma peptides afupiafupi.Mapuloteni KIli ndi mawonekedwe a catalytic triad wamba Asp39-His69-Ser224 yapadera kwa serine proteases ndipo ili ndi malo awiri omangira Ca2+ kuzungulira malo ogwirira ntchito kuti iwonjezere kukhazikika kwake ndikusunga ntchito yayikulu ya enzymatic pansi pa mikhalidwe yosiyanasiyana.
1. Zida zodziwira matenda a majini.
2. Zida zochotsera RNA ndi DNA.
3. Chotsani zinthu zopanda mapuloteni m'thupi ndikuwononga zinthu zodetsa mapuloteni (monga kukonzekera katemera wa DNA ndi heparin).
4. Kukonzekera DNA ya Chromosomal kuti igwiritsidwe ntchito pa Pulse Electrophoresis.
5. Kufalikira kwa Western.
6. Kupanga ndi kupanga ma enzyme glycated albumin reagents m'munda wa in vitro diagnostics.
1. 30mg/botolo 1g/botolo kapena 100g/botolo
2. 1kgs/thumba la zojambulazo
Proteinase K ndi protease yokhazikika ya serine yokhala ndi mawonekedwe apadera a substrate. Imawononga mapuloteni ambiri omwe ali m'malo mwake ngakhale pakakhala sopo. Siimagwira ntchito ndi ma ayoni achitsulo, ma chelating agents (monga EDTA), ma sulfhydryl reagents, kapena ndi tripsin kapena chymotrypsin inhibitors. Ndi yokhazikika pa pH yochuluka (4–12.5), yokhala ndi ntchito yabwino kwambiri pa pH 6.5–9.5. Ntchito imatha kuyambitsidwa powonjezera ma denaturing agents (SDS ndi Urea). Denaturation yofulumira ya enzyme imachitika kutentha kopitilira 70°C. Autolysis ya enzyme imachitika kwambiri pa alkaline pH. Komabe, Proteinase K siigwira ntchito kwathunthu ndi autolysis. Zidutswa zina za enzyme zimapitirizabe kugwira ntchito yawo yonse ya proteolytic, ngakhale pambuyo pa autolysis yayikulu. Proteinase K imagwiritsidwa ntchito nthawi zambiri mu ma molecular biology kuti idye mapuloteni osafunikira, monga ma nucleases mu DNA kapena RNA preparations ochokera ku tizilombo toyambitsa matenda, maselo obzalidwa, ndi zomera. Enzymeyi nthawi zambiri imagwiritsidwa ntchito pa 50–200 ug/ml mu nucleic acid yokonzekera pa pH 7.5–8.0 ndi 37~55oC. Nthawi yofukizira imasiyana kuyambira mphindi 30 mpaka maola 18.
Proteinase K imagwiritsidwa ntchito poyeretsa RNA ndi DNA kuchokera ku minofu kapena maselo.
Membala aliyense wa gulu lathu lalikulu la ndalama zogwirira ntchito amayamikira zosowa za makasitomala ndi kulumikizana kwa bungwe kuti apeze Proteinase K CAS yogulitsidwa kwambiri padziko lonse lapansi.39450-01-6Ndi chiwopsezo chachikulu cha kufalikira kwa zinthu komanso tsiku lomaliza la kupanga, pakadali pano, tikuyembekezera mgwirizano waukulu ndi makasitomala akunja kutengera phindu lomwe tonse tili nalo. Onetsetsani kuti simukulipira chilichonse kuti mulumikizane nafe kuti mudziwe zambiri.
Zogulitsidwa kwambiri m'fakitaleChina 39450-01-6， Tikulandirani ndi manja awiri thandizo lanu ndipo tidzatumikira makasitomala athu kunyumba ndi kunja ndi zinthu zapamwamba kwambiri komanso ntchito yabwino kwambiri yogwirizana ndi chitukuko chamtsogolo monga mwa nthawi zonse. Tikukhulupirira kuti mudzapindula ndi ukatswiri wathu posachedwa.
